生理学实验指南及医学研究基础

　　《生理学实验指南及医学研究基础》采用五章的篇幅统括了现代生理学实验教材的必要内容。*章阐述了生理学实验的要求、学习目的和意义；第二章精选了14个经典的动物实验；第三章选择了12个人体实验；第四、第五章简要介绍了医学科学研究的基础知识与实验设计的基本原理和原则，并提供了实验报告书写和研究论文发表的资料。这样的编排反映了作者在书中构思和内容选择上的精雕细琢，颇具匠心。在保留了生理学实验的精华内容的基础上，作者还在书中每个实验后面增加了相关问题，为学生提供拓展性学习和研究性学习的空间。此外，书中还介绍了与实验相关的“诺贝尔生理学或医学奖”的核心内容，集知识性、趣味可读性和科学实用性于一体。这有助于培养学生科学思维能力和人文精神，也是该书为突出的优点。

　　王跃春，血液内科学博士，暨南大学基础医学院生理学系副教授，硕士生导师，主要研究方向为神经生理与临床以及干细胞生理与临床。2010-2012年在香港大学李嘉诚医学院外科学系、2013-2015年在美国加州罗马琳达大学生理学系及神经生物学研究室做访问学者。广东省“生理学精品课程”“双语生理学网络课程”“广东省生理学教学团队项目”以及教育部“来华留学优质示范课程生理学”的核心成员。在教学方面，主持完成“人体生理学”和“诺贝尔奖解析一医学篇”两项优质示范课程，广受好评；开设通识教育核心课程“诺贝尔奖解析一医学篇”。以作者发表SCI论文8篇。主持各类科研课题8项。编写出版《感觉器官生理学》《环境生理学》、Clinical Respiratory Medicine. Peptide Nanofiber Scaold for Brain Tissue Reconstruction等多本著作和教材。

序
Preface
ChapterⅠ Introduction to Physiological Experiments
Section 1 General course description of physiological experiments
Section 2 Laboratory rules and requirements of physiological experiments
Section 3 The development of physiolopcal experiment and basic types of physiological experiment
Section 4 Preparation for common reagents used in physiological laboratory
Section 5 Formats and requirements of physiological experimental reports
Chapter11 Animal Experiments
Section 1 Basic technique for amphibian animal experiments
Section 2 Basic technique for mammal experiments
Section 3 Basic technique for rodent animal experiments
Section 4 BL -420 biological data acquisition and analysis system
Section 5 Specific animal experiments
Experiment 1 Sciatic-gastroc：nemius preparation
Experiment 2 Threshold， supra-threshold and maximal stimulus
Experiment 3 Single muscle twitch， frequency summation， and tetanization
Experiment 4 Action potential of nerve trunk
Experiment 5 Conduction velocity of compound AP of nerve trunk
Experiment 6 The pacemaker of frog heart
Experiment 7 Extrasystole and compensatory pause of the frog heart
Experiment 8 Osmotic fragility of the red blood corpuscles
Experiment 9 Regulation of respiration movement
Experiment 10 Regulation of cardiovascular system
Experiment 11 Physiological properties of smooth muscle of digestive tract
Experiment 12 Factors affecting urinary formation
Experiment 13 The reflex arc
Experiment 14 Ataxia induced by cerebellar injury in mouse
ChapterⅡ Human Experiments
Section 1 Introduction to WebChart -400 system for human experiments
Section 2 Specific human experiments
Experiment 1 Recording of human respiratory movement
Experiment 2 Detection of heart sounds
Experiment 3 Measurement of blood pressure
Experiment 4 Recording of an electrocardiogram
Experiment 5 Determination of ABO blood types
Experiment 6 Examination of visual acuity
Experiment 7 Examination of visualfields
Experiment 8 Examination of blind spot
Experiment 9 Examination of color blindness
Experiment 10 Conduction ways of sound
Experiment 11 Induction of knee jerk reflex
Experiment 12 Human auditory and visual responses
ChapterⅢ Fundamentals of Medical Research
Section 1 Scientific method and experimental medicine
Section 2 Basic principles of experimental medicine
Section 3 Guidelines to write and publish a research paper
Chapter V ExperimentaI Design
Section 1 Introduction to experimental design
Section 2 Methods for designing a good physiological experiment
Section 3 An example of physiological experimental design
Section 4 An example of a full lab report
Section 5 Suggested topics for physiological experimental design
Appendix
Appendix 1 Laboratory syllabus for physiological experiment（Applicable to clinical medicr
Appendix 2 Laboratory syllabus for human physiological experiment（Applicable to phamacology
Appendix 3 Grade table for examination in physiological experiments
Appendix 4 An example of physiological experimental report
Ac knowle ge ment
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