书籍详情
现代组织化学技术和方法
作者：周劲松 编
出版社：西安交通大学出版社
出版时间：2015-04-01
ISBN：9787560555614
定价：¥39.80
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内容简介
《现代组织化学技术和方法/研究生创新教育系列教材》的主要内容包括组织标本的前期制备技术和方法，组织化学基本概念和理论以及糖、核酸、脂和酶的染色方法，免疫组织化学基本概念和理论以及染色方法，组织化学与免疫组织化学染色技术与凝集素染色技术、PCR、原位杂交和电镜技术的结合。在《现代组织化学技术和方法/研究生创新教育系列教材》最后，着重介绍了目前比较先进的定量检测仪器的基本理论和使用方法，以及与本领域有关的最近研究进展。
作者简介
暂缺《现代组织化学技术和方法》作者简介
目录
Chapter 1 Introduction
Section 1 The contents and theories in Histochemistry
Section 2 The basic requirements of Histochemistry methods
Chapter 2 The Tissue Preparation
Section 1 Tissue collection
Section 2 Fixation
Section 3 Tissue washing-up， dehydration and clearance
Section 4 Embedding
Section 5 Sectioning
Section 6 Adherence and mounting
Section 7 Buffer
Chapter 3 The Carbohydrate and its Derivatives Histochemistry
Section 1 Classification
Section 2 Histochemistry methods
Chapter 4 The Nucleic Acid Histochemistry
Section 1 DNA Histochemical demonstration
Section 2 Comparison demonstration of DNA and RNA Histochemistry
Chapter 5 The Lipid Histochemistry
Section 1 Overview
Section 2 Demonstration of lipids with physical methods
Section 3 Demonstration of lipids with chemical methods
Chapter 6 Enzyme Histochemistry
Section 1 Enzyme and its basic histochemical theory
Section 2 Histochemistry for common enzymes
Chapter 7 The Basic Theory of Immunohistochemistry
Section 1 Basic Immunology
Section 2 Common markers and their detection
Section 3 Basic conditions
Chapter 8 Common Methods Used in Immunohistochemistry
Section 1 Principles
Section 2 Immunofluorescence method
Section 3 Immunoenzyme technique
Section 4 Avidin-biotin method
Section 5 Protein A method
Section 6 Immunogold method and immuno gold-silver method
Chapter 9 Specificity and Sensitivity of Immunohistochemistry
Section 1 Specificity and specific staining
Section 2 Control experiment
Section 3 The methods to enhance immunohistochemical sensitivity
Chapter 10 The Double-Staining in Immunohistochemistry
Section 1 Double-staining Immunohistochemistry on serial section
Section 2 Immunofluorescence double labeling technique
Section 3 Immunoenzyme double staining technique
Section 4 Immunoenzyme-Immunofluorescence double staining
Section 5 Immunoenzyme-Immunogold double staining
Chapter 11 The Lectin Histochemistry
Section 1 Characteristics and application of lectin
Section 2 Application of lectin Histochemistry
Chapter 12 The Progress of in Situ Display
Section 1 The envision method
Section 2 The catalyzed signal amplification method
Section 3 The ferric oxide alternative method for HRP
Chapter 13 In Situ Hybridization Immunohistochemistry
Section 1 Basic principle
Section 2 Probe preparation
Section 3 Procedure of in situ hybridization histochemistry
Section 4 Factors affecting in situ hybridization
Section 5 Control test
Chapter 14 In Situ Polymerase Chain Reaction Technique
Section 1 Basic principle
Section 2 Basic types
Section 3 Procedure
Section 4 Application of in situ PeR technology
Chapter 15 Electron Microscopic Cytochemical Technique
Section 1 Electron microscopic enzyme cytochemistry technology
Section 2 Electron microscopic Immunocytochemical technique
Chapter 16 The Quantitative Assay of Histochemistry Results
Section 1 The photomicrography
Section 2 The image analyzer
Section 3 The flow cytometry
Section 4 The laser scanning confocal microscopy
References
Appendix I Preparation of Commonly Used Buffer
Appendix II Histochemistry and Immunohistochemistry Experiments
Appendix III Figures and pictures
Section 1 The contents and theories in Histochemistry
Section 2 The basic requirements of Histochemistry methods
Chapter 2 The Tissue Preparation
Section 1 Tissue collection
Section 2 Fixation
Section 3 Tissue washing-up， dehydration and clearance
Section 4 Embedding
Section 5 Sectioning
Section 6 Adherence and mounting
Section 7 Buffer
Chapter 3 The Carbohydrate and its Derivatives Histochemistry
Section 1 Classification
Section 2 Histochemistry methods
Chapter 4 The Nucleic Acid Histochemistry
Section 1 DNA Histochemical demonstration
Section 2 Comparison demonstration of DNA and RNA Histochemistry
Chapter 5 The Lipid Histochemistry
Section 1 Overview
Section 2 Demonstration of lipids with physical methods
Section 3 Demonstration of lipids with chemical methods
Chapter 6 Enzyme Histochemistry
Section 1 Enzyme and its basic histochemical theory
Section 2 Histochemistry for common enzymes
Chapter 7 The Basic Theory of Immunohistochemistry
Section 1 Basic Immunology
Section 2 Common markers and their detection
Section 3 Basic conditions
Chapter 8 Common Methods Used in Immunohistochemistry
Section 1 Principles
Section 2 Immunofluorescence method
Section 3 Immunoenzyme technique
Section 4 Avidin-biotin method
Section 5 Protein A method
Section 6 Immunogold method and immuno gold-silver method
Chapter 9 Specificity and Sensitivity of Immunohistochemistry
Section 1 Specificity and specific staining
Section 2 Control experiment
Section 3 The methods to enhance immunohistochemical sensitivity
Chapter 10 The Double-Staining in Immunohistochemistry
Section 1 Double-staining Immunohistochemistry on serial section
Section 2 Immunofluorescence double labeling technique
Section 3 Immunoenzyme double staining technique
Section 4 Immunoenzyme-Immunofluorescence double staining
Section 5 Immunoenzyme-Immunogold double staining
Chapter 11 The Lectin Histochemistry
Section 1 Characteristics and application of lectin
Section 2 Application of lectin Histochemistry
Chapter 12 The Progress of in Situ Display
Section 1 The envision method
Section 2 The catalyzed signal amplification method
Section 3 The ferric oxide alternative method for HRP
Chapter 13 In Situ Hybridization Immunohistochemistry
Section 1 Basic principle
Section 2 Probe preparation
Section 3 Procedure of in situ hybridization histochemistry
Section 4 Factors affecting in situ hybridization
Section 5 Control test
Chapter 14 In Situ Polymerase Chain Reaction Technique
Section 1 Basic principle
Section 2 Basic types
Section 3 Procedure
Section 4 Application of in situ PeR technology
Chapter 15 Electron Microscopic Cytochemical Technique
Section 1 Electron microscopic enzyme cytochemistry technology
Section 2 Electron microscopic Immunocytochemical technique
Chapter 16 The Quantitative Assay of Histochemistry Results
Section 1 The photomicrography
Section 2 The image analyzer
Section 3 The flow cytometry
Section 4 The laser scanning confocal microscopy
References
Appendix I Preparation of Commonly Used Buffer
Appendix II Histochemistry and Immunohistochemistry Experiments
Appendix III Figures and pictures
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