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DNA芯片：芯片平台和湿实验方法（A辑 导读版）
作者：（美）基梅尔、等
出版社：科学出版社
出版时间：2007-01-01
ISBN：9787030182272
定价：¥90.00
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内容简介
《DNA芯片》共两册，汇集了DNA芯片技术领域各个环节的专家，对DNA芯片技术进行了一次全方位的介绍，兼顾实用性和学术性。读者将可从中获得关于DNA芯片技术具体而实用的操作指导，在DNA芯片实验中少走弯路。两本书的重要特点包括：（1）系统性。由于DNA芯片技术是在融合了多门学科的基础上产生的交叉学科，涉及了学、精密仪器、电子微加工、化学、生物学、生物信息学等学科的内容，因此本书汇集了各个技术环节的专家，各自编写自己擅长的部分。并且在章节的结构安排上，是先进行全面的介绍，然后深入到具体环节，逐一进行详细的阐述。例如先从整体上介绍DNA芯片技术的现状，包括一些主要的商业化DNA芯片技术平台，然后引伸到研究者若要自己构建DNA芯片技术平台，需要具体注意哪些事项。（2）继承性。DNA芯片技术实际上根植于传统的分子生物学。本书在介绍DNA芯片技术实验方法时，多借用分子生物学家业已熟悉的传统方法，从这些方法的基本原理入手，过渡到DNA芯片技术的操作中。例如，书中交待了有哪些原理和方法与传统方法类似，而又有哪些环节不同，在操作的时候是需要着重注意的，让即使还没有接触过DNA芯片技术的人也不会感到陌生。（3）实用性。针对分子生物学学的需求进行安排，使分子生物学家能够很容易地领悟和掌握芯片杂交实验操作和数据处理的技巧，使DNA芯片实验设计，实验实施，到论文发表的整个过程简便易行。（4）前瞻性。对许多新技术进行了介绍，以开拓读者的视野，引导读者将DNA芯片技术向各个应用领域延伸。本套书适合于从事生物芯片研发应用以及分子生物学、生物化学、细胞生物学、免疫学、生物信息学、生物技术、生物工程、蛋白质组学、基因组学等生命科学相关研究领域的教学科研人员、亦可供生物技术企业的研发者和决策者参考使用。
作者简介
暂缺《DNA芯片：芯片平台和湿实验方法（A辑 导读版）》作者简介
目录
Section Ⅰ Array Platforms
1 The Affymetrix GeneChip Platform:An Overview
2 The Agilent In Situ Synthesized Microarray Platform
3 Illumina Universal Bead Arrays
4 Microarray Oligonucleotide-probes
5 Automated Liquid Handling and High-Throughput Preparation of Polymerase Chain Reaction Amplified DNA for Microarray Fabrication
6 The Printing Process:Tips on Tips
7 Making and Using Spotted DAN Microarrays in an Academic Core Laboratory
8 Printing Your Own Inkjet Microarrays
9 Peptide Nucleic Acid Microarrays Made with(S,S)-trans-Cyclopentane-Constrained Peptide Nucleic Acids
Section Ⅱ Wet-Bench Protocols
10 Optimizing Experiment and Analysis Parameters for Spotted Microarrays
11 Sample Labeling:An Overview
12 Genomic DNA as a General Cohybridization Standard for Ratiometric Microarrays
13 Analysis of Sequence Specificities of DNA-Binding Proteins with Protein Binding Microarrays
14 Microarray Analysis of RNA Processing and Modification
15 Mapping the Distribution of Chromatin Proteins by ChIP on Chip
16 DamID:Mapping of In Vivo Protein-Genome Interactions Using Tethered DNA Adenine Methyltransferase Using Tethered DNA-Adenine Methyltransferase
17 Whole-Genome Genotyping
18 Mapping Drosophila Genomic Aberration Breakpoints with Comparative Genome Hybridization on Microarrys
19 Performing Quantitative Reverse-Transcribed Polymerase Chain Reaction Experiments
20 The Application of Tissue Microarrays in the Validation of Microarray Results
21 Mapping Histone Modefications by Nucleosome Immunoprecipitation
AUTHOR INDEX
SUBJECT INDEX
1 The Affymetrix GeneChip Platform:An Overview
2 The Agilent In Situ Synthesized Microarray Platform
3 Illumina Universal Bead Arrays
4 Microarray Oligonucleotide-probes
5 Automated Liquid Handling and High-Throughput Preparation of Polymerase Chain Reaction Amplified DNA for Microarray Fabrication
6 The Printing Process:Tips on Tips
7 Making and Using Spotted DAN Microarrays in an Academic Core Laboratory
8 Printing Your Own Inkjet Microarrays
9 Peptide Nucleic Acid Microarrays Made with(S,S)-trans-Cyclopentane-Constrained Peptide Nucleic Acids
Section Ⅱ Wet-Bench Protocols
10 Optimizing Experiment and Analysis Parameters for Spotted Microarrays
11 Sample Labeling:An Overview
12 Genomic DNA as a General Cohybridization Standard for Ratiometric Microarrays
13 Analysis of Sequence Specificities of DNA-Binding Proteins with Protein Binding Microarrays
14 Microarray Analysis of RNA Processing and Modification
15 Mapping the Distribution of Chromatin Proteins by ChIP on Chip
16 DamID:Mapping of In Vivo Protein-Genome Interactions Using Tethered DNA Adenine Methyltransferase Using Tethered DNA-Adenine Methyltransferase
17 Whole-Genome Genotyping
18 Mapping Drosophila Genomic Aberration Breakpoints with Comparative Genome Hybridization on Microarrys
19 Performing Quantitative Reverse-Transcribed Polymerase Chain Reaction Experiments
20 The Application of Tissue Microarrays in the Validation of Microarray Results
21 Mapping Histone Modefications by Nucleosome Immunoprecipitation
AUTHOR INDEX
SUBJECT INDEX
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