书籍详情
从基因到基因组：DNA技术概念和应用
作者：（美）J.W.戴尔（Jeremy W.Dale），（美）M.冯尚茨（Malcolm Von Schantz）著
出版社：科学出版社
出版时间：2004-01-01
ISBN：9787030124777
定价：¥46.00
购买这本书可以去
内容简介
基因技术、遗传工程、重组DNA和基因克隆等技术的快速发展，把分子生物学推到了生命科学的前沿。本书对分子生物学核心技术的原理及其使用进行了简明扼要的阐释。以对分子生物学的基本概念的简短介绍开篇，然后介绍核心的分子生物学方法及其整合运用，从单个基因的克隆与研究到整个基因组的测序，以及基因组信息的分析。最后，本书还介绍了这些技术在生物技术、医药和农业以及整个生命科学研究中的应用。＜br＞本书内容涵盖了分子生物学基础、基因克隆、核酸的纯化与分离、DNA的剪切与连接、载体、基因组文库和cDNA文库、聚合酶链反应、DNA测序、序列数据分析、基因变异分析、基因表达分析、基因功能分析、医药应用、转基因等等方面。＜br＞本书适于生物化学、分子生物学、遗传学、细胞生物学、生物技术、生物工程等相关学科及研究领域的本科生、研究生及教学科研人员参考使用。
作者简介
暂缺《从基因到基因组：DNA技术概念和应用》作者简介
目录
Preface
1 Introduction
2 Basic Molecular Biology
2.1 Nucleic Acid Structure
2.2 Gene Structure and Organization
2.3 Information Flow:Gene Expression
3 How to Clone a Gene
3.1 What is Cloning?
3.2 Wverview of the Procedures
3.3 Gene Libraries
3.4 Hybridization
3.5 Polymerase Chain Reaction
4 Purification and Separation of Nucleic Acids
4.1 Extraction and Purification of Nucleic Acids
4.2 Detection and Quantitation of Nucleic Acids
4.3 Gel Electrophoresis
5 Cutting and Joining DNA
5.1 Restriction Endonucleases
5.2 Ligation
5.3 Alkaline Digests
5.4 Double Digests
5.5 Modification of Restriction Fragment Ends
5.6 Other Ways of Joining DNA Molecules
5.7 Summary
6 Vectors
6.1 Plasmid Vectors
6.2 Vectors Based on the Lambda Bacteriophage
6.3 Cosmids
6.4 M13 Vectors
6.5 Expression Vectors
6.6 Vectors for Cloning and Expression in Eukaryotic Cells
6.7 Supervectors:YACs and BACs
6.8 Summary
7 Genomic and cDNA Libraries
7.1 Genomic Libraries
7.2 Growing and Storing Libraries
7.3 cDNA Libraries
7.4 Random,Arrayed and Ordered Libraries
8 Finding the Right Clone
8.1 Screening Libraries with Gene Probes
8.2 Screening Expression Libraries with Antibodies
8.3 Rescreening
8.4 Subcloning
8.5 Characterization of Plasmid Clones
9 Polymerase Chain Reaction(PCR)
9.1 The PCR Reaction
9.2 PCR in Practice
9.3 Cloning PCR Products
9.4 Long-range PCR
9.5 Reverse-transcription PCR
9.6 Rapid Amplification of cDNA Ends(RACE)
9.7 Applications of PCR
10 DNA Sequencing
10.1 Principles of DNA Sequencing
10.2 Automated Sequencing
10.3 Extending the Sequence
10.4 Shotgun Sequencing:Contig Assembly
10.5 Genome Sequencing
11 Analysis of Sequence Data
11.1 Analysis and Annotation
11.2 Databanks
11.3 Sequence Comparisons
12 Analysis of Genetic Variation
12.1 Nature of Genetic Variation
12.2 Methods for Studying Variation
13 Analysis of Gene Expression
13.1 Analysing Transcription
13.2 Comparing Transcriptomes
13.3 Methods for Studying the Promoter
13.4 Translational Analysis
14 Analysis of Gene Function
14.1 Relating Genes and Functions
14.2 Genetic Maps
14.3 Relating Genetic and Physical Maps
14.4 Linkage Analysis
14.5 Transposon Mutagenesis
14.6 Allelic Replacement and Gene Knock-outs
14.7 Complementation
14.8 Studying Gene Function through Protein Interactions
15 Manipulating Gene Expression
15.1 Factors Affecting Expression of Clonde Genes
15.2 Expression of Cloned Genes in Bacteria
15.3 Expression in Eukaryotic Host Cells
15.4 Adding Tags and Signals
15.5 In vitro Mutagenesis
16 Medical Applications,Present and Future
16.1 Vaccines
16.2 Detection and Identification of Pathogens
16.3 Human Genetic Diseases
17 Transgenics
17.1 Transgenesis and Cloning
17.2 Animal Transgenesis and its Applications
17.3 Transgenic Plants and their Applications
17.4 Summary
Bibliography
Glossary
Index
1 Introduction
2 Basic Molecular Biology
2.1 Nucleic Acid Structure
2.2 Gene Structure and Organization
2.3 Information Flow:Gene Expression
3 How to Clone a Gene
3.1 What is Cloning?
3.2 Wverview of the Procedures
3.3 Gene Libraries
3.4 Hybridization
3.5 Polymerase Chain Reaction
4 Purification and Separation of Nucleic Acids
4.1 Extraction and Purification of Nucleic Acids
4.2 Detection and Quantitation of Nucleic Acids
4.3 Gel Electrophoresis
5 Cutting and Joining DNA
5.1 Restriction Endonucleases
5.2 Ligation
5.3 Alkaline Digests
5.4 Double Digests
5.5 Modification of Restriction Fragment Ends
5.6 Other Ways of Joining DNA Molecules
5.7 Summary
6 Vectors
6.1 Plasmid Vectors
6.2 Vectors Based on the Lambda Bacteriophage
6.3 Cosmids
6.4 M13 Vectors
6.5 Expression Vectors
6.6 Vectors for Cloning and Expression in Eukaryotic Cells
6.7 Supervectors:YACs and BACs
6.8 Summary
7 Genomic and cDNA Libraries
7.1 Genomic Libraries
7.2 Growing and Storing Libraries
7.3 cDNA Libraries
7.4 Random,Arrayed and Ordered Libraries
8 Finding the Right Clone
8.1 Screening Libraries with Gene Probes
8.2 Screening Expression Libraries with Antibodies
8.3 Rescreening
8.4 Subcloning
8.5 Characterization of Plasmid Clones
9 Polymerase Chain Reaction(PCR)
9.1 The PCR Reaction
9.2 PCR in Practice
9.3 Cloning PCR Products
9.4 Long-range PCR
9.5 Reverse-transcription PCR
9.6 Rapid Amplification of cDNA Ends(RACE)
9.7 Applications of PCR
10 DNA Sequencing
10.1 Principles of DNA Sequencing
10.2 Automated Sequencing
10.3 Extending the Sequence
10.4 Shotgun Sequencing:Contig Assembly
10.5 Genome Sequencing
11 Analysis of Sequence Data
11.1 Analysis and Annotation
11.2 Databanks
11.3 Sequence Comparisons
12 Analysis of Genetic Variation
12.1 Nature of Genetic Variation
12.2 Methods for Studying Variation
13 Analysis of Gene Expression
13.1 Analysing Transcription
13.2 Comparing Transcriptomes
13.3 Methods for Studying the Promoter
13.4 Translational Analysis
14 Analysis of Gene Function
14.1 Relating Genes and Functions
14.2 Genetic Maps
14.3 Relating Genetic and Physical Maps
14.4 Linkage Analysis
14.5 Transposon Mutagenesis
14.6 Allelic Replacement and Gene Knock-outs
14.7 Complementation
14.8 Studying Gene Function through Protein Interactions
15 Manipulating Gene Expression
15.1 Factors Affecting Expression of Clonde Genes
15.2 Expression of Cloned Genes in Bacteria
15.3 Expression in Eukaryotic Host Cells
15.4 Adding Tags and Signals
15.5 In vitro Mutagenesis
16 Medical Applications,Present and Future
16.1 Vaccines
16.2 Detection and Identification of Pathogens
16.3 Human Genetic Diseases
17 Transgenics
17.1 Transgenesis and Cloning
17.2 Animal Transgenesis and its Applications
17.3 Transgenic Plants and their Applications
17.4 Summary
Bibliography
Glossary
Index
猜您喜欢
